polyvinylidene difluoride membranes (Bio-Rad)

Bio-Rad is a verified supplier

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Bio-Rad polyvinylidene difluoride membranes
Polyvinylidene Difluoride Membranes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 2910 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyvinylidene+difluoride+membranes/Trans-Blot+Turbo+Mini+PVDF+Transfer+Packs/pmc13018931-112-23-27
Average 96 stars, based on 2910 article reviews

polyvinylidene difluoride membranes - by Bioz Stars, 2026-09

96/100 stars

Images

Western Blot:

Article Title: Effects of music on hepatic cytochrome P450 1A enzymes in Sprague Dawley rats.
Article Snippet: All rat-specific qPCR primers (Table 1) were synthesized and supplied by Integrated DNA Technologies (Coralville; IA; USA), and their specificity was verified using the Primer-BLAST tool (Primer-BLAST, 2026). .. Western blotting reagents included 2× Laemmli Sample Buffer (catalog# 1610737) and the Trans-Blot Turbo RTA Transfer Kit which includes the polyvinylidene difluoride membranes (catalog# 1704272) from Bio-Rad Laboratories (Hercules, CA, USA). .. Primary monoclonal antibodies against CYP1A1 (catalog# sc-393,979) and CYP1A2 (catalog# sc53,241) were obtained from Santa Cruz Biotechnology (Dallas, TX, USA), while the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (catalog# ab8245) was sourced from Abcam (Cambridge, UK).

Nucleic Acid Electrophoresis:

Article Title: MYC-bound enhancer RNAs in cis regulate gene transcription and tumorigenesis
Article Snippet: The total protein was quantified by a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, 23225) and boiled at 95°C for 10 min with an equal volume of 2× Laemmli Sample Buffer (Bio-Rad, 161-0737). .. About 30 μg of proteins was resolved by SDS–polyacrylamide gel electrophoresis under denaturing conditions and transferred to polyvinylidene difluoride membranes (Bio-Rad, 162-0177). .. Membranes were blocked for 1 hour with 5% nonfat milk (LabScientific, M0841) in PBST solution at room temperature and incubated with a diluted primary antibody at 4°C overnight, washed three times with PBST, and incubated with a diluted horseradish peroxidase–conjugated goat anti-mouse IgG (Invitrogen, 31430) or goat anti-rabbit IgG (Invitrogen, 31460) secondary antibody for 1 hour at room temperature.

Article Title: Comparative proteomic profiling of receptor kinase signaling reveals key trafficking components enforcing plant stomatal development
Article Snippet: After an additional 1-hour incubation at 26°C, chemiluminescence signals were measured using an EnVision 2105 multimode plate reader (Revvity). .. Protein samples were resolved on 8 to 10% SDS–polyacrylamide gel electrophoresis gels and transferred to polyvinylidene difluoride membranes (BioRad catalog no. 1620177). .. Membranes blocked in 5% nonfat milk in Tris-buffered saline with Tween 20 for 1 hour at room temperature, followed by overnight incubation at 4°C with primary antibodies, including anti-HA (1:5000, Abcam, catalog no. Ab18181), anti-GFP (1:5000, Invitrogen, catalog no. 33-2600), anti-RFP (1:2000, ChromoTek, catalog no. pabr1), anti-FLAG (1:5000, Sigma-Aldrich, catalog no. F3165), and anti-ACTIN (1:10,000, Abcam, catalog no. Ab230169 ).

Article Title: Endothelial Nitric Oxide Synthase Restores Diabetic Dentin Regeneration via AKT/Endothelial Nitric Oxide Synthase Axis
Article Snippet: Protein concentration was determined using a bicinchoninic acid assay kit (P0012, Beyotime). .. Twenty micrograms of protein from each sample were separated by 4% or 15% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (4561083, Bio-Rad) and transferred to polyvinylidene difluoride membranes (1704156, Bio-Rad). .. After blocking with 5% BSA, the membranes were incubated overnight at 4°C with primary antibodies against: DSPP (1:2000, bs-10316R, Bioss), DMP1 (1:1000, 12359R, Bioss), p-AKT (1:2000, 4060S, CST), AKT (1:500, 60203-2-Ig, Proteintech), eNOS (1:500, ab76198, Abcam), and GAPDH (1:5000, AC026, Proteintech).

Polyacrylamide Gel Electrophoresis:

Article Title: Porphyromonas gingivalis outer membrane vesicles promote alveolar bone resorption by increasing the local RANKL/OPG ratio in experimental periodontitis model rats.
Article Snippet: .. Briefly, equal amounts of proteins (30 μg) were denatured and separated via 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride membranes (catalog numbers 4561096 and 1704156; Bio-Rad). ..

SDS Page:

Article Title: Targeting circSFPQ_008/SFPQ/BRCA1 axis for overcoming platinum resistance in ovarian cancer.
Article Snippet: Platinum resistance is the main cause of treatment failure in ovarian cancer.. BRCA1/2-mediated DNA damage repairment is an important factor contributing to platinum resistance in ovarian cancer.. Altering the expression levels of BRCA1/2 will affect the platinum sensitivity of ovarian cancer.

Article Title: Peptide YY reduces cytotoxicity of Candida albicans in alcohol-associated liver disease
Article Snippet: .. The proteins were resolved on polyacrylamide gels by SDS-PAGE (Bio-rad), and then transferred to polyvinylidene difluoride membranes (1620175, Bio-rad). .. Immunoblot analysis was performed using anti-gasdermin D (cleaved and full-length peptides) (1:1,000) (MA5-44666, ThermoFisher), anti-IL1b (cleaved form) (1:1,000) (ab9722, Abcam) and anti-GAPDH (1:5000) (GTX627408, GeneTex).

Blocking Assay:

Article Title: Class I histone deacetylases and their inhibitors as targets to modulate cellular senescence in osteoarthritis.
Article Snippet: For Western blotting, 100 μg protein lysate was prepared with 1 × NuPAGE (Invitrogen, Cat. No. NP0007) supplemented with 1 × sample reducing agent (Life Technologies, Cat. No. B0009) which was boiled at 95 °C for 5 min. .. Equal amounts of proteins were separated by SDS‐PAGE and transferred onto polyvinylidene difluoride membranes (BIO-RAD; Trans-Blo Turbo Mini PVDF Transfer Packs; Cat. No. 1704156) which were blocked for 1 h with blocking buffer (LI-COR, Cat. No. 927–60,001). .. Primary antibodies included: HDAC1 (Thermo Fisher Scientific, Cat. No. PA1-860; 1:1000), HDAC2 (Cell Signaling Technology, Cat. No. 57156; 1:1000), HDAC3 (Cell Signaling Technology, Cat. No. 3949; 1:1000), HDAC8 (Cell Signaling Technology, Cat. No. 66042; 1:1000), Acetyl-Histone H3 (Cell Signaling Technology, Cat. No. 4353; 1:500), Histone H3 (Cell Signaling Technology, Cat. No. 9715; 1:1000), cleaved PARP (Cell Signaling Technology, Cat. No. 5625; 1:100), PARP (Cell Signaling Technology, Cat. No. 9542; 1:1000), and GAPDH (Thermo Fisher Scientific, Cat. No. AM4300; 1:2000), Bax6A7 (Santa Cruz, Cat. No. 23959; 1:100), Bax (Cell Signaling Technology, Cat. No. 5023; 1:1000), Bcl-XL (Cell Signaling Technology, Cat. No. 2764; 1:1000), antirabbit (Licor, Cat. No. 926–32,211), and anti-mouse (Licor, Cat. No. 926–68,070).

Review